Method for increasing the content of docosahexaenoic acid in...

C - Chemistry – Metallurgy – 12 – P

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C12P 7/64 (2006.01) C11C 3/00 (2006.01) C12N 1/14 (2006.01)

Patent

CA 2676556

[PROBLEMS] To provide an oil-and-fat having an increased docosahexaenoic acid content. [MEANS FOR SOLVING PROBLEMS] Disclosed are: a method for producing a highly unsaturated fatty acid, which is characterized by culturing a stramenopile capable of producing the highly unsaturated fatty acid in a culture medium containing a substance which inhibits a fatty acid desaturase; a highly unsaturated fatty acid, particularly an oil-and-fat having an increased docosahexaenoic acid content, which is produced by the method; a method for enhancing the ability of producing a highly unsaturated fatty acid of a stramenopile, which is characterized by culturing the stramenopile in a culture medium containing a substance which inhibits a fatty acid desaturase; and a stramenopile having an enhanced ability of producing a highly unsaturated fatty acid, which is produced by the method. Particularly, in the method for producing the highly unsaturated fatty acid, the method for enhancing the ability of producing the highly unsaturated fatty acid, and the stramenopile having an enhanced ability of producing the highly unsaturated fatty acid, the stramenopile may be a microorganism belonging to the class Labyrinthulomycetes.

L'invention vise à proposer une huile-et-graisse ayant une teneur accrue en acide docosahexaénoïque. A cet effet, l'invention porte sur un procédé de production d'un acide gras hautement insaturé qui est caractérisé par la mise en culture d'un straménopile capable de produire l'acide gras hautement insaturé dans un milieu de culture contenant une substance qui inhibe une acide gras désaturase ; sur un acide gras hautement insaturé, en particulier une huile-et-graisse ayant une teneur accrue en acide docosahexaénoïque, qui est obtenu par le procédé ; sur un procédé pour augmenter l'aptitude à produire un acide gras hautement insaturé d'un straménopile qui est caractérisé par la mise en culture du straménopile dans un milieu de culture contenant une substance qui inhibe une acide gras désaturase ; et sur un straménopile ayant une aptitude accrue à produire l'acide gras hautement insaturé qui est obtenu par le procédé. En particulier, dans le procédé de production de l'acide gras hautement insaturé, le procédé pour augmenter l'aptitude à produire l'acide gras hautement insaturé et le straménopile ayant une aptitude accrue à produire l'acide gras hautement insaturé, le straménopile peut être un microorganisme appartenant à la classe Labyrinthulomycetes.

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