Method for increasing the content of polyunsaturated...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C11B 3/00 (2006.01) C11C 1/08 (2006.01) C12N 9/04 (2006.01) C12N 9/20 (2006.01) C12N 9/88 (2006.01) C12N 15/53 (2006.01) C12N 15/55 (2006.01) C12N 15/60 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2573972

The invention relates to a method for increasing the content of polyunsaturated long-chained fatty acids in an organism, by introducing nucleic acid coding for polypeptides and/or proteins having phopholipase, ketoacyl-CoA-reductase and/or dehydratase activity into the organisms. Advantageously, said enzymes stem from ostreococcus or thraustochytrium. The invention also relates to nucleic acid sequences, nucleic acid structures, vectors and organisms containing the inventive nucleic acid sequences, vectors containing the nucleic acid sequences and/or the nucleic acid structures, and transgenic organisms containing the above-mentioned nucleic acid sequences, nucleic acid structures and/or vectors. Advantageously, said above-mentioned nucleic acid frequencies, nucleic acid structures and vectors can optionally be expressed in the organism along with additional nucleic acid sequences coding for the polypeptides and/or proteins of fatty acids or lipid metabolism biosynthesis. Another advantage of the nucleic acid sequences of the fatty acids or lipid metabolism is that they are coded for .DELTA.-9 elongase, .DELTA.-8-desaturase, a .DELTA.-5-desaturase, .DELTA.-4-desaturase, .DELTA.-12- desaturase, .DELTA.-5-elongase and/or .DELTA.-6-elongase activity. Said desaturases and elongases stem from organisms such as thalassiosira, euglena, isochrysis, physcomitrella, thraustochytrium, borago, phytophthora, crypthecodinium, oncorhynchus, primula, xenopus, ciona, arabidopsis, mortierella, caenorhabditis, phaeodactylum, ceratodon or ostreococcus. The invention further relates to oils, lipids, and/or fatty acids that are produced according the inventive method, and to the use thereof, in addition to the inventive unsaturated fatty acids and triglycerides having an increased content of unsaturated fatty acids and to the use thereof.

L'invention concerne un procédé permettant d'augmenter la teneur d'un organisme en acides gras à chaîne longue polyinsaturés. Selon ce procédé, on introduit dans l'organisme des acides nucléiques qui sont codants pour les polypeptides ou protéines ayant une activité de phospholipase, cétoacyl-CoA-réductase et/ou déshydratase. De préférence, les enzymes sont issus d'ostreococcus ou de thraustochytrium. L'invention concerne également les séquences d'acides nucléiques, les structures d'acides nucléiques, les vecteurs et organismes contenant les séquences d'acides nucléiques selon l'invention, les vecteurs contenant les séquences d'acides nucléiques et/ou les structures d'acides nucléiques ainsi que les organismes transgéniques contenant les séquences d'acides nucléiques, les structures d'acides nucléiques et/ou les vecteurs précités. De préférence, les séquences d'acides nucléiques, les structures d'acides nucléiques et les vecteurs précités peuvent, éventuellement avec d'autres séquences d'acides nucléiques qui sont codantes pour des polypeptides ou protéines de la biosynthèse du métabolisme des acides gras ou des lipides, être exprimés dans l'organisme. Des séquences d'acides nucléiques particulièrement avantageuses du métabolisme des acides gras ou des lipides sont codantes pour une activité de .DELTA.-9 élongase, .DELTA.-8-désaturase, .DELTA.-5-désaturase, .DELTA.-4-désaturase, .DELTA.-12-désaturase, .DELTA.-5-élongase et/ou .DELTA.-6-élongase. De préférence, ces désaturases et élongases proviennent des organismes suivants Thalassiosira, Euglena, Isochrysis, Physcomitrella, Thraustochytrium, Borago, Phytophthora, Crypthecodinium, Oncorhynchus, Primula, Xenopus, Ciona, Arabidopsis, Mortierella, Caenorhabditis, Phaeodactylum, Ceratodon ou Ostreococcus. Un autre aspect de l'invention concerne les huiles, lipides et/ou les acides gras produits d'après ce procédé et leur utilisation. L'invention concerne enfin des acides gras insaturés et des triglycérides ayant une teneur accrue en acides gras insaturés et leur utilisation.

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