Method for increasing the expression of polypeptides in...

C - Chemistry – Metallurgy – 12 – N

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195/1.36

C12N 5/10 (2006.01) C12N 15/67 (2006.01) C12N 15/85 (2006.01)

Patent

CA 1333778

Viable cell proportions and total cell densities in recombinant eukaryotic cell cultures are increased by transfecting a eukaryotic host cell with nucleic acid encoding a desired polypeptide and with an oncogene. Transfectant cultures are screened thereafter for the expression of elevated quantities of the desired polypeptide as compared to expression from the parent host cell.

616540

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