Method for increasing the growth of plant cell cultures

Details Method for increasing the growth of plant cell cultures Method for increasing the growth of plant cell cultures

C - Chemistry, Metallurgy

12

N

C12N 5/04 (2006.01) C12N 5/00 (2006.01) C12P 1/00 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2278588

The present invention relates to a method for increasing the growth rate and concentration of in vitro cultivated plant cells by re-induction and stimulation of the growth of plant cells, which comprises the steps of: a) growing plant cell cultures in a nutrient medium under growth conditions suitable for initiation of growth; and b) supplementing cell culture with additional macronutrients at any time between an initial growing stage and before culture death in an amount sufficient to re-induce growth without being toxic to the culture; whereby the growth is re-induced, stimulated, maintained and increased for obtaining increased plant cell concentration in culture.

Cette invention a trait à une méthode permettant d'accélérer la vitesse de croissance de cellules végétales cultivées et d'augmenter leur concentration et ce, in vitro, par ré-activation et stimulation du développement desdites cellules. Cette méthode consiste, (a), à faire pousser des cultures de cellules végétales dans un milieu nutritif dans des conditions favorables au démarrage d'une croissance, (b), à apporter à la culture cellulaire, à n'importe quel moment entre sa phase initiale de croissance et la période précédant sa mort, des polynutriments complémentaires et ce, sous des quantités suffisantes pour réactiver la croissance mais dépourvues de toxicité à l'encontre de la culture. Du fait de ces opérations, la croissance est réactivée, stimulée, amenée à perdurer et renforcée et ce, dans le but d'accroître la concentration de cellules végétales dans la culture.

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