Method for increasing the production of physiologically...

C - Chemistry – Metallurgy – 12 – P

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C12P 21/00 (2006.01) C12N 1/38 (2006.01) C12N 5/00 (2006.01) C12N 9/72 (2006.01) C12N 15/67 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2324385

The production of a physiologically active substance by a normal, transfected or transformed cultured cell can be increased by culturing the cell in a culture medium containing cobalt and molybdenum. The culture medium is one ordinarily selected for growing those types of cells which produce a desired, physiologically active substance, e.g., a recombinant growth factor of a monoclonal antibody. When cobalt and molybdenum are included in any of these growth media, a substantial improvement in the production of a physiologically active substance by the cells can be observed. Compositions and methods utilizing cobalt and molybdenum as additives for culture media are described.

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