Method for increasing thermostability in cellulase emzymes...

Details Method for increasing thermostability in cellulase emzymes... Method for increasing thermostability in cellulase emzymes...

C - Chemistry, Metallurgy

12

N

C12N 15/56 (2006.01) C12N 9/42 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2198828

The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product.

Le gène codant l'endoglucanase EI d'Acidothermus cellulolyticus est cloné et exprimé dans Pichia pastoris. Une nouvelle enzyme endoglucanase E1 modifiée comprenant le domaine catalytique de l'enzyme E1 pleine taille présente une plus grande thermostabilité et est produite au moyen de deux méthodes. La première méthode de production de la nouvelle E1 modifiée est un clivage protéolytique pour éliminer le domaine de liaison de la cellulose et le lieu peptide de la E1 pleine taille. La seconde méthode de production de la nouvelle E1 modifiée consiste en une troncation génétique deu gène codant la E1 pleine taille de façon à exprimer le domaine catalytique dans le produit d'expression.

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