C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/63 (2006.01) A61K 31/713 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01)
Patent
CA 2543002
The present invention relates to a method for inducing an activity of a specific RNAi in eukaryotic cells, into which the TAT protein and a nucleic acid are introduced, said nucleic acid coding for a nucleotide sequence which comprises the sense and antisense sequences of an RNAi of interest. Said sense and antisense sequences are separated by a separating nucleotide sequence, coding for a sequence which comprises at least the minimum TAR sequence which is recognised by the TAT protein, said transcribed separating sequence and the TAT protein forming a complex which inhibits the activity of the RNAi of interest. The RNAi activity is then induced, by removing the TAT protein.
La présente invention a pour objet une méthode d~induction d~une activité d~un ARNi spécifique dans des cellules eucaryotes dans lesquelles on introduit la protéine TAT et un acide nucléique codant pour une séquence nucléotidique comprenant les séquences sens et antisens d~un ARNi d~intérêt, lesdites séquences sens et antisens étant séparées par une séquence nucléotidique séparatrice codant pour une séquence comprenant au moins la séquence TAR minimale reconnue par la protéine TAT, ladite séquence séparatrice transcrite et la protéine TAT formant un complexe inhibant l~activité de l~ARNi d~intérêt et on induit l~activité de l~ARNi en retirant la protéine TAT.
Ait-Si-Ali Slimane
Benkirane Monsef
Harel-Bellan Annick
Centre National de La Recherche Scientifique - Cnrs
Gowling Lafleur Henderson Llp
LandOfFree
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