Method for inducing osteoblast differentiation of human...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/077 (2010.01) A61K 35/12 (2006.01) A61K 35/32 (2006.01)

Patent

CA 2309907

A method for inducing osteoblast differentiation and/or for obtaining cells engaged in osteoblast differentiation, starting from human extramedullary adipose tissue cells, comprising the step consisting in incubating said starting cells in a liquid nutrient medium for a period of time sufficient to enable said cells to develop, said nutrient medium containing a solution of at least one glucocorticoid and being free from adipogenic factor. Applicable to making bone implants, in particular using autologous cells.

Procédé pour induire une différenciation ostéoblastique et/ou pour obtenir des cellules engagées dans la différenciation ostéoblastique, au départ de cellules de tissu adipeux extramédullaire humain, comprenant l'étape consistant à faire incuber pendant un temps suffisant lesdites cellules de départ dans un milieu nutritif liquide permettant le développement desdites cellules, ledit milieu nutritif contenant en solution au moins un glucocorticoïde et étant exempt de facteur adipogénique. Application à la réalisation d'implants osseux, en particulier à l'aide de cellules autologues.

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