Method for introducing antisense oligonucleotides into...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/88 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2427068

The present invention relates to a method for introducing one or more antisense oligonucleotides into one or more eucaryotic cells using one or more lipid formulations comprising one or more cationic lipids of Formula I and optionally at least one neutral lipid. In particular, the present invention relates to a method for introducing one or more antisense oligonucleotides into one or more eucaryotic cells using a lipid formulation comprising dimethyldioctadecylammonium bromide (DDAB) and at least one neutral lipid, especially dioleylphosphatidylethanolamine (DOPE). The invention also relates to kits for carrying out the invention, compositions for carrying out the invention, and compositions formed while carrying out the invention. Further, the present invention relates to a method for inhibiting or preventing cell growth or proliferation, and a method for inhibiting or preventing expression of one or more proteins.

La présente invention porte sur un procédé d'introduction d'un ou plusieurs oligonucléotides antisens dans une ou plusieurs cellules eucaryotes au moyen d'une ou plusieurs formulations lipidiques comprenant un ou plusieurs lipides cationiques de la formule (I) et éventuellement au moins un lipide neutre. Cette invention porte notamment sur un procédé d'introduction d'un ou plusieurs oligonucléotides antisens dans une ou plusieurs cellules eucaryotes au moyen d'une formulation lipidique comprenant un bromure diméthyldioctadécylammonium (DDAB) et au moins un lipide neutre, notamment dioléylphosphatidyléthanolamine (DOPE). L'invention porte aussi sur des kits de réalisation, sur des compositions pour la réalisation de l'invention et sur des compositions obtenues au moyen de cette invention. L'invention porte, en outre, sur un procédé d'inhibition ou de prévention de la prolifération cellulaire, et sur un procédé d'inhibition ou de prévention de l'expression d'une ou plusieurs protéines.

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