C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.17, 195/1.
C12N 15/00 (2006.01) C12N 5/02 (2006.01) C12N 15/85 (2006.01) C12P 21/00 (2006.01)
Patent
CA 1214127
ABSTRACT OF THE DISCLOSURE Multiple copies of a foreign DNA I coding for the production of a protein may be introduced into eucaryotic cells by cotransforming suitable cells with foreign DNA I and with foreign DNA II which includes a functionally deficient, amplifiable gene coding for a selectable or identifiable trait, preferably carried on the same DNA molecule as a foreign DNA III, which includes a functional, amplifiable gene coding for another selectable or identifiable trait. The cotransformation is carried out under suitable conditions permitting selection or identification of cells expressing the gene on DNA I or that on DNA III, but not that on DNA II. The cotrans- formed cells so identified or selected are recovered and cloned under conditions where the functionally deficient gene on DNA II is expressed. Cells expressing the gene on DNA II are recovered. They contain multiple copies of DNA I. This method can be used to produce mRNA tran- scripts or protein products such as human and animal growth hormone, insulin and the like.
423503
Axel Richard
Roberts James M.
Ridout & Maybee Llp
The Trustees Of Columbia University
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