C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/87 (2006.01) A61K 31/70 (2006.01) A61K 38/16 (2006.01) A61K 48/00 (2006.01) C07K 14/075 (2006.01) C07K 14/47 (2006.01) C07K 14/54 (2006.01) C12N 5/10 (2006.01) C12N 15/34 (2006.01) C12N 15/79 (2006.01) C12N 15/85 (2006.01)
Patent
CA 2190963
The toxicity problems arising when foreign matter is introduced into higher eukaryotic cells, especially with transfection with DNA, are obviated by expression in the cell of gene products that block the apoptosis induced by the transfection process and/or by treating the cells with anti-inflammatory substances. Preferred anti-apoptosis genes are Bc1-2, E1B 19K or an anti-apoptotic gene of the CELO avian adenovirus; used as preferred anti-inflammatory substance in the adenovirus VA1, which is introduced into the cell in the form of VA1 DNA. These measures help to achieve a long-lasting gene expression.
Lors de l'introduction d'un matériel génétique étranger dans des cellules eucaryotes d'ordre supérieur, notamment lors de la transfection par ADN, des problèmes de toxicité interviennent. Afin de les éviter, des produits géniques sont exprimés dans la cellule et bloquent l'apoptose induite par le processus de transfection, et/ou les cellules sont traitées à l'aide de substances anti-inflammatoires. On utilise de préférence comme gènes anti-apoptose le Bcl-2, l'E1B 19K ou un gène de l'aviadénovirus CELO à effet anti-apoptique, et comme substance à effet anti-inflammatoire, l'adénovirus VA1 introduit sous forme d'ADN-VA1 dans la cellule. Ces mesures permettent de parvenir à une expression génique durant longtemps.
Baker Adam
Chiocca Susanna
Cotten Matthew
Boehringer Ingelheim International Gmbh
Fetherstonhaugh & Co.
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