Method for introducing nucleic acid in higher eukaryotic cells

Details Method for introducing nucleic acid in higher eukaryotic cells Method for introducing nucleic acid in higher eukaryotic cells

C - Chemistry, Metallurgy

12

N

C12N 15/87 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2191762

The invention pertains to a method and medium for transfection of higher eukaryotic cells with DNA-polycation complexes wherein the medium contains ethyleneglycol and/or glycerine. In addition, the medium optionally contains a substance that prevents acidification of the endosomes, and/or a lower alcohol such as ethanol. The method is especially suitable for transfection of primary cells such as fibroblasts. This yields stably transfected cells, for example tumor cells to be used as tumor vaccines.

L'invention concerne un procédé et un milieu servant à la transfection de cellules eucaryotes d'ordre supérieur à l'aide de complexes ADN/polycation, le milieu contenant de l'éthylèneglycol et/ou de la glycérine. Le milieu contient en outre une substance qui empêche l'acidification des endosomes, et/ou un alcool inférieur tel que de l'éthanol. Ce procédé s'utilise tout particulièrement dans la transfection de cellules primaires, telles que des fibroblastes, et permet d'obtenir des cellules transfectées de manière stable, par ex. des cellules tumorales s'utilisant comme vaccins antitumoraux.

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