Method for isolating a target biological material, capture...

G - Physics – 01 – N

Patent

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G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2263756

The invention concerns a method for isolating a target biological material contained in a sample, which consists in providing a capture phase comprising an organic molecule having at least a reactive function and at least a protein material capable of recognising or binding, specifically and directly or indirectly, with the target biological material, said protein material having a specific covalent binding site with the organic molecule reactive function, consisting of at least a tag comprising at least six contiguous lysine, or lysine derivative residues; the method consists in contacting said target biological material with at least the capture phase; and detecting the target biological material fixed on the capture phase: The invention also concerns the capture and detection phases, and a reagent containing them.

L'invention concerne un procédé de mise en évidence d'un matériel biologique cible contenu dans un échantillon, selon lequel on dispose d'une phase de capture qui comprend une molécule organique ayant au moins une fonction réactive et au moins un matériel protéique susceptible de reconnaître ou de se lier, spécifiquement et directement ou indirectement, au matériel biologique cible, ledit matériel protéique possédant un site spécifique de liaison par covalence à la fonction réactive de la molécule organique, qui consiste en au moins un tag comprenant au moins six résidus lysine, ou dérivé de lysine, contigus; on met en contact ledit matériel biologique cible avec au moins la phase de capture; et on détecte le matériel biologique cible fixé sur la phase de capture. L'invention concerne aussi des phases de capture et de détection, et un réactif les contenant.

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