Method for isolating and purifying grass pollen allergens

Details Method for isolating and purifying grass pollen allergens Method for isolating and purifying grass pollen allergens

C - Chemistry, Metallurgy

07

K

C07K 14/415 (2006.01) A61K 39/36 (2006.01) G01N 33/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2381340

The invention relates to a method for quickly and effectively isolating and purifying five, namely the group 1, 2, 3, 10 and 13 allergens from grass pollen. The purification of said grass pollen is based on the inventive combination of hydrophobic interaction chromatography, gel filtration and cation exchange chromatography. The proteins obtained by the inventive method facilitate an improved diagnosis of pollen allergies and are used in pharmaceutical preparations for the therapy of pollenogenic diseases.

L'invention concerne un procédé d'isolation et de purification rapides et efficaces de cinq allergènes des groupes 1, 2, 3, 10, et 13 des pollens de plantes herbacées. La purification repose sur une combinaison selon l'invention entre chromatographie par interaction hydrophobe, filtration de gel, et chromatographie par échange de cations. Les protéines ainsi obtenues peuvent être utilisées pour un diagnostic amélioré d'allergies aux pollens, et dans des préparations pharmaceutiques destinées à la thérapie de maladies liées aux allergies aux pollens.

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