Method for isolating ligands

C - Chemistry – Metallurgy – 07 – K

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C07K 7/06 (2006.01) A61K 35/14 (2006.01) A61K 38/08 (2006.01) A61K 39/245 (2006.01) A61P 31/12 (2006.01) C07K 7/08 (2006.01) C07K 14/045 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2476571

Described is a method for isolating ligands which have a binding capacity to a MHC/HLA molecule or a complex comprising said ligand and said MHC/HLA molecule which method comprises the following steps: - providing a pool of ligands, said pool containing ligands which bind to said MHC/HLA molecule and ligands which do not bind to said MHC/HLA molecule, - contacting said MHC/HLA molecule with said pool of ligands whereby a ligand which has a binding capacity to said MHC/HLA molecule binds to said MHC/HLA molecule and a complex comprising said ligand and said MHC/HLA molecule is formed, - detecting and optionally separating said complex from the ligands which do not bind to said MHC/HLA molecule and - optionally isolating and characterising the ligand from said complex as well as a method for isolating T cell epitopes which have a binding capacity to a MHC/HLA molecule.

Cette invention concerne une méthode permettant d'isoler des ligands présentant une capacité de liaison à la molécule MHC/HLA ou un complexe composé de ce ligand et de cette molécule MHC/HLA, laquelle méthode comprend les étapes suivantes consistant : à utiliser un ensemble de ligands, lequel ensemble comprend des ligands qui se lient à la molécule MHC/HLA ainsi que des ligands qui ne se lient pas à cette molécule MHC/HLA ; à mettre en contact cette molécule MHC/HLA avec l'ensemble de ligands, après quoi un ligand présentant une capacité de liaison à la molécule MHC/HLA se lie à cette molécule MHC/HLA, ce qui permet d'obtenir un complexe composé du ligand et de la molécule MHC/HLA ; à détecter et éventuellement à séparer ce complexe des ligands qui ne se lient pas à cette molécule MHC/HLA ; et éventuellement à isoler et à caractériser le ligand de ce complexe. Cette invention concerne également une méthode permettant d'isoler des épitopes de lymphocyte T qui présentent une capacité de liaison à la molécule MHC/HLA.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Method for isolating ligands does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for isolating ligands, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for isolating ligands will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1725072

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.