Method for isolating mb creatine kinase

Details Method for isolating mb creatine kinase Method for isolating mb creatine kinase

C - Chemistry, Metallurgy

12

Q

195/123, 403/2,

C12Q 1/50 (2006.01) C12N 9/12 (2006.01)

Patent

CA 1130229

ABSTRACT OF THE DISCLOSURE Creatine kinase MB isoenzyme can be seperated from a mixture of creatine kinase isoenzymes by ion exchange chromatography. Use of a mixture of cation and anion ex- change resin in this method permits elution of the MB iso- enzyme from the mixed resin while the other isoenzymes are wholely or partially retained. In a preferred embodiment the mixed resin is a mixture of a weakly basic anion exchange resin and a weakly acidic cation exchange resin.

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