Method for isolating nucleic acids

Details Method for isolating nucleic acids Method for isolating nucleic acids

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01)

Patent

CA 2622037

Described herein is a method in which genomic nucleic acid of a cell can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid (e.g., plasmid DNA) of the cell directly from a cell growth culture. Also described herein, a method in which genomic nucleic acid can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid in a cell lysate without the need to prepare a cleared lysate. The present invention is also directed to a method of isolating genomic nucleic acid (e.g., RNA, DNA) of a cell or an organism.

La présente invention a trait à un procédé dans lequel l'acide nucléique génomique d'une cellule peut être séparé de l'acide nucléique ayant un poids moléculaire qui est inférieur au poids moléculaire de l'acide nucléique génomique (par exemple, l'ADN plasmidique) de la cellule directement à partir d'une culture cellulaire. L'invention a également trait à un procédé dans lequel l'acide nucléique génomique peut être séparé de l'acide nucléique ayant un poids moléculaire qui est inférieur au poids moléculaire de l'acide nucléique génomique dans un lysat cellulaire sans nécessiter la préparation d'un lysat clarifié. La présente invention a trait en outre à un procédé d'isolement de l'acide nucléique génomique (par exemple, l'ARN, l'ADN) d'une cellule ou d'un organisme.

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