A - Human Necessities – 01 – N
Patent
A - Human Necessities
01
N
A01N 1/02 (2006.01) A61K 35/52 (2006.01) A61P 19/00 (2006.01) C12N 5/08 (2006.01)
Patent
CA 2617541
The invention relates to a method for isolating multipotent stem cells from dental tissue, according to which the stem cells are obtained from a tissue composite and are subsequently cultivated. The invention also relates to stem cells that have been isolated using said method and to osteocytes and neuronal cells, which have been obtained by means of said method. The invention further relates to a method for producing a bank of stem cells, according to which the cells thus obtained are stored. According to the invention, the cells of cushion-type soft tissue, which can be localised beneath the papilla on the apical side of an extracted immature tooth, are cryopreserved in a tissue composite. The tissue composite is only broken down to obtain the stem cells after being defrosted. The results indicate that stem or progenitor cells can be isolated even after the cryopreservation of the original tissue and that said stem or progenitor cells react to osteogenic stimulation. In addition, the cells react to a greater extent after cryopreservation than without the latter.
L'invention concerne un procédé pour isoler des cellules souches multipotentes à partir d'un tissu dentaire, les cellules souches étant obtenues puis cultivées à partir d'un ensemble tissulaire. L'invention concerne également des cellules souches isolées selon ce procédé, ainsi que des cellules osseuses et des cellules neuronales produites selon ledit procédé. La présente invention porte également sur un procédé pour réaliser une banque de cellules souches, selon lequel les cellules sont stockées selon le procédé de l'invention. Selon l'invention, les cellules d'un tissu mou de type coussinet, qui peut être localisé directement sur la face apicale d'une dent immature extraite sous la papille, sont cryoconservées dans l'ensemble tissulaire, lequel n'est désagrégé qu'après décongélation pour obtenir les cellules souches. Les résultats montrent que, même après cryoconservation du tissu original, il est possible d'isoler des cellules souches/précurseurs qui réagissent à une stimulation ostéogénique. En outre, les cellules réagissent plus après cryoconservation que sans cryoconservation.
Degistirici Oezer
Thie Michael
Cassan Maclean
Stiftung Caesar
LandOfFree
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