Method for isolation of recombinant polypeptides in...

Details Method for isolation of recombinant polypeptides in... Method for isolation of recombinant polypeptides in...

C - Chemistry, Metallurgy

07

K

530/3.08, 195/1.

C07K 14/43 (2006.01) C07K 1/113 (2006.01)

Patent

CA 1265461

"METHOD FOR ISOLATION OF RECOMBINANT POLYPEPTIDES IN BIOLOGICALLY ACTIVE FORM" ABSTRACT Disclosed are improved methods for orienting polypeptide molecules containing multiple disulfide bonds into their native conformations through (1) treating the polypeptide with (a) a denaturing agent and (b) a reducing agent capable of reductively dissociating cystine disulfide bonds; (2) concurrently reducing the concentration of the reducing agent while forming a stable intermediate by reacting a disulfide containing compound with the reduced cysteine moeities of the polypeptide and; (3) forming native cystine disulfide bonds in the presence of a mild oxidizing/reducing environment while dissociating the disulfide-group polypeptide moeities.

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