Method for labeling polynucleotide sequences

Details Method for labeling polynucleotide sequences Method for labeling polynucleotide sequences

C - Chemistry, Metallurgy

12

N

195/1.12

C12N 15/09 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1295559

ABSTRACT A method for labeling a polynucleotide sequence to form a polynucleotide probe is disclosed. The method comprises forming a modified polynucleotide sequence wherein the modification comprises the cleavage of preselected bases to generate sugar aldehydes. The preselected bases are either quaternized nitrogen purines or uracil. A signalling moiety is attached to the modified polynucleotide sequence by means of the sugar aldehydes.

515507

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