Method for ligating nucleic acids and molecular cloning

C - Chemistry – Metallurgy – 12 – N

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C12N 15/66 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2401632

The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.

L'invention porte sur des procédés visant à assembler de manière covalente des molécules d'acide nucléique et sur des procédés de clonage moléculaire. Ces procédés consistent à effectuer une ligature séquentielle ou simultanée de molécules flanquantes ou vectorielles d'acide nucléique et des molécules d'insertion d'acide nucléique par topoisomérase ou ligase de l'ADN. Ces procédés permettent de réaliser l'assemblage covalent directionnel et non directionnel et le clonage des molécules d'acide nucléique.

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