Method for lysing cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/06 (2006.01) C12N 1/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2212446

This invention relates to a method for lysing cells. The method comprises simultaneously flowing a cell suspension and a lysis solution through a static mixer, wherein the cells exit the static mixer lysed. In another aspect of the present invention, the invention relates to a method for precipitating cell components, protein, and nucleic acids from a cell lysate or other solution containing precipitable material. The method comprises simultaneously flowing a cell lysate or other protein containing solution and precipitating solution through a static mixer, wherein the lysate or protein solution exits the static mixer with its precipitable components precipitated. In another aspect of the present invention, the invention relates to a method where the two above- mentioned methods are combined by using static mixers in series.

La présente invention concerne une méthode de lyse de cellules. Cette méthode comprend l'écoulement simultané d'une suspension de cellules et d'une solution de lyse dans un mélangeur statique, duquel les cellules sortant lysées. Un autre aspect de la présente invention concerne une méthode de précipitation des constituants cellulaires, des protéines et des acides nucléiques à partir d'un lysat de cellules ou d'une autre solution contenant une matière pouvant être précipitée. Cette méthode consiste à faire s'écouler simultanément un lysat de cellules ou autre solution contenant des protéines et une solution de précipitation dans un mélangeur statique, duquel le lysat ou la solution de protéines sort avec tous ses éléments pouvant être précipités ayant précipité. Un autre aspect de la présente invention concerne une méthode consistant à combiner les deux méthodes susmentionnées en utilisant des mélangeurs statiques en série.

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