C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/02 (2006.01) C07K 14/52 (2006.01) C12N 5/06 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2205430
A method of producing a natural cytokine mixture is disclosed including the steps of immobilizing at least one mitogen in a tissue culture vessel. An isolated population of lymphocytes free of neutrophils and erythrocytes, is suspended in a serum-free media and placed in the vessel. The lymphocytes are cultured, the media removed, and characterized for the yield of cytokines.
La présente invention concerne un procédé de production d'un mélange de cytokine naturelle. Ce procédé consiste à immobiliser au moins un mitogène dans un bac de culture tissulaire. Le procédé consiste ensuite à mettre en suspension dans un milieu non sérique une population de lymphocytes exempts de neutrophiles et d'érythrocytes et à les placer dans le bac. Le procédé consiste enfin à mettre en culture les lymphocytes, à éliminer le milieu de culture, et à classer les lymphocytes en fonction de la teneur en cytokines.
Moffat & Co.
University Of South Florida
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