Method for mammalian cell separation from a mixture of cell...

Details Method for mammalian cell separation from a mixture of cell... Method for mammalian cell separation from a mixture of cell...

C - Chemistry, Metallurgy

12

Q

C12Q 1/24 (2006.01) B03C 1/32 (2006.01) C12M 1/42 (2006.01) C12N 5/06 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2173218

The invention provides a method for both the positive and negative selection of at least one mammalian cell population from a mixture of cell populations utilizing a magnetically stabilized fluidized bed. One desirable application of this method is the separation and purification of mammalian hematopoietic cells. Target cell populations include human stem cells.

L'invention porte sur une méthode de sélection positive et négative d'au moins une population de cellules de mammifères à partir d'un mélange de populations de cellules à l'aide d'un lit fluidisé à stabilisation magnétique. L'une des applications préconisées de cette méthode consiste dans la séparation et la purification de cellules hématopoïétiques de mammifères . Les populations de cellules cibles englobent les cellules souches humaines.

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