Method for manufacturing a recombinant polyclonal protein

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/10 (2006.01) C07K 16/00 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2683800

The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a collection of variant nucleic acid sequences, wherein each cell in the collection is transfected with and capable of expressing one member of the collection, which encodes a distinct member of a polyclonal protein. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The nucleic acid sequence is introduced into the cells by transfection with a collection of vectors. The present method is suitable for manufacturing recombinant polyclonal antibodies for therapeutic uses.

L'invention porte sur un procédé de fabrication d'une composition de protéine polyclonale recombinante, en particulier d'une composition d'anticorps polyclonal recombinant. Le procédé comporte l'opération consistant à obtenir un ensemble de cellules transfectées avec un ensemble de séquences d'acide nucléique variantes, chaque cellule dans l'ensemble étant transfectée avec un élément de l'ensemble et capable d'exprimer un élément de l'ensemble qui code pour un élément distinct d'une protéine polyclonale. Les cellules sont cultivées dans des conditions appropriées pour l'expression de la protéine polyclonale qui est obtenue à partir des cellules ou d'un surnageant de culture. La séquence d'acide nucléique est introduite dans les cellules par transfection avec un ensemble de vecteurs. Le présent procédé est approprié pour fabriquer des anticorps polyclonaux recombinants pour des utilisations thérapeutiques.

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