C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/56 (2006.01) C07K 14/745 (2006.01)
Patent
CA 2448188
A method for manufacturing a tissue factor-based prothrombin-time reagent includes combining predetermined quantities of tissue factor (TF), phospholipid and detergent- containing buffer to create a TF/phospholipid mixture. In this combining step, the quantity of TF is predetermined based upon its measured TF activity. Next, the detergent is removed from the TF/phospholipid mixture to produce an essentially detergent-free TF/phospholipid mixture that is useful as a tissue factor-based PT reagent. The method can include the use of hydrophobic resin that has been prescreened based on phospholipide recovery to accomplish the removal of the detergent.
Earp Brian
Haley Paul
Jenny Richard
Lifescan Inc.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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