C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2366643
The invention relates to a Happy Mapping type method for mapping a DNA molecule comprising a step enabling ad infinitum amplification of the DNA of each panel, consisting of the following: (i) a first amplification with the aid of a primer comprising 10-30 nucleotides defined at extremity 5' thereof, and 5 -10 random nucleotides at extremity 3' thereof, in addition to (ii) a second amplification with the aid of a primer comprising at least the oligonucleotides defined at extremity 5' of the primer used in stage i).
La présente invention concerne un procédé de cartographie d'une molécule d'ADN du type Happy Mapping qui comprend une étape permettant l'amplification ad infinitum de l'ADN de chaque panel consistant en (i) une première amplification à l'aide d'une amorce comprenant de 10 à 30 nucléotides définis en son extrémité 5', et de 5 à 10 nucléotides aléatoires en son extrémité 3' et (ii) une deuxième amplification à l'aide d'une amorce comprenant au moins les oligonucléotides définis de l'extrémité 5' de l'amorce utilisée à l'étape i).
Cavaloc Yvon
Galibert Francis
Cavaloc Yvon
Centre National de La Recherche Scientifique (c.n.r.s.)
Galibert Francis
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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