Method for massive directed mutagenesis

Details Method for massive directed mutagenesis Method for massive directed mutagenesis

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2420721

The invention concerns the field of molecular biology and more particularly that of mutagenesis. It concerns a method of high-rate directed mutagenesis, that is the formation of numerous directed mutants in reduced time and with reduced number of steps. Said method is therefore referred to as massive directed mutagenesis.

La pr~sente invention concerne le domaine de la biologie mol~culaire et plus particuli~rement celui de la mutagen~se. Elle a pour objet un proc~d~ de mutagen~se dirig~e ~ haut d~bit, c'est-~-dire la constitution de nombreux mutants dirig~s en un temps et un nombre d'~tapes r~duits. Ce proc~d~ sera donc qualifi~ mutagen~se massive.

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