Method for measurement of enzyme

Details Method for measurement of enzyme Method for measurement of enzyme

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) C07C 401/00 (2006.01) C12Q 1/25 (2006.01)

Patent

CA 2308455

A method of assaying enzymes such as protease, comprising the step (1) of bringing an enzyme-containing specimen into contact with a thin film containing both of an organic compound having a visible ray absorption that varies by the reaction with an enzyme and a hydrophilic macromolecule such as gelatine and the step (2) of detecting discernible traces formed on the film by the action of the enzyme.

L'invention se rapporte à une méthode d'analyse d'enzymes tels que des protéases. Cette méthode consiste (1) à amener un échantillon contenant des enzymes en contact avec un film fin contenant à la fois un composé organique dont l'absorption du rayonnement visible est modifiée par réaction avec un enzyme, et une macromolécule hydrophile telle que la gélatine, et (2) à déceler les traces susceptibles d'être discernées formées sur le film par l'action de l'enzyme.

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