G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/573 (2006.01) C07K 16/36 (2006.01) G01N 33/577 (2006.01) G01N 33/86 (2006.01)
Patent
CA 2077906
- 44 - ABSTRACT In methods for immunologically measuring the tissue factor in a specimen in a buffer solution by immune reaction, a method for measuring the tissue factor in high sensitivity which comprises (a) using a monoclonal antibody (first antibody) speci- fically recognizing the apoprotein of the human tissue factor and immobilized on an insoluble carrier, and a labeled monoclonal antibody (second antibody) specifically recognizing the apoprotein of the human tissue factor and having a recognition site different from that by the first antibody, and (b) carrying out the immune reaction in a buffer solu- tion (i) containing in a concentration of 2 to 15 weight % at least one nonionic surfactant having an HLB (hydrophilic-lipophilic balance) value of 12 or more, and (ii) containing a protein having a molecular weight of about 16,000 to about 50,000 and an isoelec- tric point of 1.0 to 5.0, or a mixture contain- ing the protein.
Hasegawa Ryoichi
Matsuzawa Kimihiko
Smart & Biggar
Teijin Limited
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