Method for measuring activated factor vii level in a sample

Details Method for measuring activated factor vii level in a sample Method for measuring activated factor vii level in a sample

G - Physics

01

N

G01N 33/86 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2727165

The present invention relates to a method for measuring the activated factor VII level in a sample to be tested, including the steps of a) mixing said test sample with a plasma free of factor VII (FVII) and free of at least another factor selected from among factor VIII (FVIII), factor IX (FIX), and factor XI (FXI), the test sample + plasma having a final FVII + FVIIa concentration of 10 pM to 80 pM, b) adding initiating components from the thrombin generation reaction, c) obtaining a thrombogram when carrying out a thrombin generation test (TGT) on the mixture from step b), d) comparing at least one of the thrombogram parameters from step c) with a homologous parameter obtained from standard thrombograms established on the basis of standard samples, the activated factor VII level of which is known and varies with each standard sample, e) deducing, from step d), an activated factor VII level measurement in the test sample

La présente invention concerne un Procédé de mesure du taux de facteur VII activé dans un échantillon à tester, comprenant les étapes consistant à : a) mélanger ledit échantillon-test avec un plasma dépourvu en facteur VII (FVII) et dépourvu en au moins un autre facteur choisi parmi le facteur VIII (FVIII), le facteur IX (FIX) et le facteur XI (FXI), le mélange échantillon-test + plasma ayant une concentration finale en FVII +FVIIa allant de 10 pM à 80 pM; b) ajouter des composants initiateurs de la réaction de génération de thrombine; c) obtenir un thrombinogramme en effectuant un test de génération de thrombine (TGT) sur le mélange de l' étape b); d) comparer au moins l'un des paramètres du thrombinogramme de l'étape c) à un paramètre homologue obtenu à partir de thrombinogrammes standards établis sur la base d'échantillons- standards dont le taux de Facteur VII activé est connu et varie entre chaque échantillon- standard; e) déduire de l'étape d) une mesure du taux de Facteur VII activé dans l' échantillon-test.

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