Method for measuring antigens or antibodies in biological...

G - Physics – 01 – N

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167/41, 167/43,

G01N 33/543 (2006.01) G01N 33/531 (2006.01) G01N 33/538 (2006.01) G01N 33/548 (2006.01)

Patent

CA 1291028

METHOD FOR MEASURING ANTIGENS OR ANTIBODIES IN BIOLOGICAL FLUIDS USING LIGAND LABELED ANTIGENS OR LIGAND LABELED ANTIBODIES ABSTRACT OF THE DISCLOSURE A method is described for measuring the level of circulating antigens or antibodies by reacting a patient sample with a ligand labeled specific antigen or antibody carried on a liquid matrix in the presence of a differently labeled specific anti-antigen or labeled specific anti-antibody, this immuno- logical complex thus formed is reacted with an immobilized anti-ligand on a solid support which is directed against the ligand attached to the specific antigen or antibody through the liquid matrix, and subsequently the solid phase is washed and checked for the label on the anti-antigen or anti-antibody which is directly proportional to the concentration of specific antigen or antibody. A method is also described for measuring the level of circulating antigens or antibodies by reacting a patient sample with a ligand labeled specific antigen or a ligand labeled specific antibody carried on a matrix, then contacting said matrix with an immobilized anti-ligand on a solid support; washing the solid support and reacting it with a differently labeled anti-antigen or anti-antibody, followed by rewashing the solid support and checking for the presence of the label attached to the anti-antigen or anti-antibody which is directly proportional to the concentration of the specific antigen or antibody.

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