Method for measuring antithrombin activity

Details Method for measuring antithrombin activity Method for measuring antithrombin activity

C - Chemistry, Metallurgy

12

Q

C12Q 1/00 (2006.01) G01N 33/86 (2006.01)

Patent

CA 2421957

The invention provides a one-stage method of measuring antithrombin (AT) activity in a sample. In the method, a diluted sample is mixed with AT- deficient plasma containing intrinsic coagulation enzymes, an AT augmenting compound such as heparin, a phospholipid and an activator of the contact phase of the intrinsic coagulation pathway. Following addition of calcium ions, coagulation time is measured and compared to a reference standard to determine the level of AT activity in the sample.

L'invention concerne un procédé à étape unique de mesure de l'activité de l'antithrombine (AT) dans un échantillon. On mélange un échantillon dilué avec du plasma déficient en antithrombine qui renferme des enzymes de coagulation intrinsèques, un composé à capacité d'augmentation de l'antithrombine du type héparine, un phospholipide et un activateur de la phase de contact du trajet de coagulation intrinsèque. Après l'adjonction d'ions calcium, on mesure le temps de coagulation et on le compare à une norme de référence pour déterminer le niveau d'activité de l'antithrombine dans l'échantillon.

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