Method for measuring endotoxin and reagent kit for measuring...

G - Physics – 01 – N

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G01N 33/579 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2709828

A technique which is less likely to be affected by turbid or color of samples and with which prompt and simple detection or concentration measurement of endotoxin can be achieved is provided. A reagent in which proteins contained in LAL are adsorbed or bound onto fine particles dispersed in a previously prepared drug liquid is prepared, and this reagent and a sample containing endotoxin are mixed. By doing this, endotoxin acts on the proteins on the fine particles and the fine particles are associated with one another to form a large aggregate at an early stage. By optically measuring the formation of this aggregate, detection or concentration measurement of endotoxin is performed.

L'invention porte sur une technique, qui est moins susceptible d'être affectée par un trouble ou une couleur d'échantillons et avec laquelle une détection ou une mesure de concentration simple et rapide d'endotoxine peuvent être obtenues. Un réactif dans lequel des protéines contenues dans LAL sont adsorbées ou liées en fines particules dispersées dans un médicament liquide préalablement préparé, est préparé, et ce réactif et un échantillon contenant de l'endotoxine sont mélangés. En faisant cela, l'endotoxine agit sur les protéines des fines particules et les fines particules s'associent les unes avec les autres pour former un large agrégat à un stade précoce. Par une mesure optique de la formation de cet agrégat, une détection ou une mesure de concentration d'endotoxine peut être réalisée.

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