G - Physics – 01 – N
Patent
G - Physics
01
N
150/9
G01N 33/53 (2006.01) G01N 33/58 (2006.01) G01N 33/78 (2006.01)
Patent
CA 1299984
ABSTRACT A method for measuring free ligands in biological fluids in the presence of bound ligand and endogenous binding proteins, without disturbing the equilibrium between the free ligand and the protein-bound ligand, comprised of the following steps: (a) incubating a sample of biological fluid with (i) a ligand analog tracer which, due to its chemical structure, does not bind to some of the endogenous binding proteins, (ii) a specific ligand binder and (iii) specific chemical inhibitor reagents that alone or in combination inhibit the binding of the ligand analog tracer to other endogenous binding proteins; (b) separating the ligand analog tracer bound to the specific binder from unbound tracer; and (c) comparing the bound fraction in said sample to the bound fraction of a given set of known free ligand calibrators to determine the concentration of free ligand in said biological fluid.
510762
Diagnostic Products Corporation
Smart & Biggar
LandOfFree
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