G - Physics – 01 – N
Patent
G - Physics
01
N
150/11, 73/51.5
G01N 33/52 (2006.01) G01N 21/64 (2006.01)
Patent
CA 1305024
ABSTRACT The invention relates to an improved method for the measurement of polarized emissions in which background emissions are compensated for without the necessity of separating a fluorescing material from a substrate which contributes the background fluorescence. The method is particularly useful in measuring fluorescence from a suspension of cells. The method involves the measurement of polarized fluorescence emissions in a vertical and a horizontal plane at a first wavelength and at a second wavelength. The second wavelength is selected based upon the bathochromic shift of the spectrum of the fluorescence emission of the background fluorescence as compared to the fluorescence of the material being analyzed at each of the wavelengths. From these measurements a factor representing the fraction of the total intensity of fluorescence emission due to background fluorescence is determined from which the intensity of the vertically and horizontally polarized emission intensities dua to background fluorescence is derived. The derived polarization emission intensities are then deducted from the vertically and horizontally polarized emission intensities obtained at the first wavelength to obtain polarized emission intensities due solely to the material being analyzed. The method of the present invention is particularly adapted for the measurement of polarized fluorescent emissions from suspensions of cells in a substrate which, which substrate contributes background fluorescence.
538175
Cercek Boris
Cercek Lea
Cercek Boris
Cercek Lea
Macrae & Co.
LandOfFree
Method for measuring polarized fluorescence emissions does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for measuring polarized fluorescence emissions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for measuring polarized fluorescence emissions will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1248155