Method for measuring substance having affinity

Details Method for measuring substance having affinity Method for measuring substance having affinity

G - Physics

01

N

G01N 33/543 (2006.01) G01N 15/02 (2006.01) G01N 15/12 (2006.01)

Patent

CA 2529362

A binding reaction of a substance having affinity, which is a substance to be measured, with a binding partner having a binding affinity with the above substance having affinity is measured through agglutination reaction. Carrier particles having the binding partner bound thereto are bound with the substance having affinity in an electric field, and the level of carrier particles agglutinated is evaluated by the numeration using three-dimensional information of the particles as indicators. The use of the three-dimensional information as indicators allows the detection or measurement of the presence of a biologically specific reactive substance, with more ease and simplicity with greater speed and also with higher sensitivity, as compared to a conventional measuring method.

Selon l'invention, une réaction de liaison d'une substance à affinité, qui est une substance à mesurer, avec un partenaire de liaison présentant une affinité de liaison avec ladite substance à affinité, est mesurée par réaction d'agglutination. Des particules porteuses présentant un partenaire de liaison fixé dessus, sont liées à la substance à affinité dans un champ électrique et le niveau de particules porteuses agglutinées est évalué par numération, à l'aide de données tridimensionnelles sur les particules, comme indicateurs. L'utilisation de données tridimensionnelles comme indicateurs permet de détecter ou de mesurer la présence d'une substance réactive spécifique sur le plan biologique, plus aisément et plus simplement, de même que plus rapidement et avec une plus grande sensibilité, comparativement à un procédé de mesure traditionnel.

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