Method for measuring the activity of deacetylase and method...

Details Method for measuring the activity of deacetylase and method... Method for measuring the activity of deacetylase and method...

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) C12Q 1/34 (2006.01) C12Q 1/48 (2006.01)

Patent

CA 2392711

The acetylation level of a peptide is evaluated by taking advantage of the fact that a change in the acetylation level affects the peptidase sensitivity of a substrate peptide. This method is usable in measuring the activity of a deacetylase or an acetylase. It is also possible to screen a substance affecting the activity of these enzymes thereby. Thus, the activity of a deacetylase can be conveniently measured.

On évalue le niveau d'acétylation d'un peptide en tirant profit du fait qu'une modification du niveau d'acétylation affecte la sensibilité à la peptidase d'un peptide de substrat. Ce procédé peut être utilisé pour mesurer l'activité d'une désacétylase ou d'une acétylase. Il est également possible de cribler une substance modifiant l'activité de ces enzymes. Ainsi, l'activité d'une désacétylase peut être mesurée de façon appropriée.

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