Method for measuring the activity of the blood clotting...

G - Physics – 01 – N

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G01N 33/86 (2006.01) C12Q 1/56 (2006.01)

Patent

CA 2422523

The invention relates to a method for measuring the activity of the blood clotting factor XIIIa. The aim of the invention is to provide a method for measuring the activity of FXIIIa which is simple to carry out and which is also suitable for use with testing systems that have a high throughput. It was found that activated FXIII influences the light transmitting power of fibrin clots. Fibrinogen is caused to clot with a fibrinogen-splitting enzyme. The change in the light transmitting power of the samples according to the concentration of FXIIIa present is registered and is used as a measure of the activity of FXIIIa. The inventive method can be used especially for screening for FXIIIa inhibitors.

Méthode de mesure de l'activité du facteur de coagulation XIIIa. L'objet de la présente invention est la mise au point d'une méthode facilement applicable de mesure de l'activité du FXIIIa, appropriée également pour être utilisée dans des systèmes d'analyse à haut rendement. Contre toute attente, il a été découvert que le FXIII activé influence la perméabilité à la lumière des caillots de fibrine. Le fibrinogène est amené à coaguler sous l'effet d'une enzyme clivant le fibrinogène. La modification de la perméabilité à la lumière des échantillons en fonction de la concentration du FXIIIa présent est enregistrée et sert de mesure pour l'activité du FXIIIa. La méthode selon la présente invention est destinée à être utilisée tout particulièrement pour cribler des inhibiteurs de FXIIIa.

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