G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/86 (2006.01)
Patent
CA 2673623
The invention relates to a method for measuring in vitro or ex vivo the concentration of FVII in a sample, that comprises the following steps: mixing said sample with human plasma free of FVII and of at least another factor selected from FVIII, FIX and FXI; b) adding compounds that initiate the thrombine generation reaction, including a calcium ion source, a phospholipidic agent and a tissular factor; c) carrying out a thrombine generation test (TGT) on the reaction medium thus obtained in order to a provide a thrombinogram with parameters; d) comparing at least one of the parameters of the thrombinogram with a homologous parameter of standard thrombinograms, each standard thrombinogram being obtained with a fixed reference concentration of FVIIa in said reaction medium in a range of 1 pM to 5 nM; and e) deducing from step d) a measure of the concentration of FVIIa in the sample included in said range.
La présente invention concerne un procédé de mesure in vitro ou ex-vivo de la concentration de FVII dans un échantillon, comprenant les étapes consistant à : a) mélanger ledit échantillon avec un plasma humain dépourvu de FVII et d'au moins un autre facteur choisi parmi le FVIII, le FIX et le FXI; b) ajouter des composants initiateurs de la réaction de génération de thrombine comprenant une source d'ions calcium, un agent phospholipidique et du facteur tissulaire; c) effectuer un test de génération de thrombine (TGT) sur le milieu réactionnel ainsi obtenu afin d'obtenir un thrombinogramme fournissant des paramètres; d) comparer au moins l'un des paramètres du thrombinogramme à un paramètre homologue de thrombinogrammes standards, chaque thrombinogramme standard étant obtenu avec une concentration étalon fixée de FVIIa dans ledit milieu réactionnel, comprise dans une plage allant de 1 pM à 5 nM, et e) déduire de l'étape d) une mesure de la concentration de FVIIa de l'échantillon comprise dans ladite plage.
Grenier Dominique
Hilbert Lysiane
Mazurier Claudine
Gowling Lafleur Henderson Llp
Lfb Biotechnologies
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