Method for measuring the concentration of transient...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/37 (2006.01) C12Q 1/56 (2006.01)

Patent

CA 2668356

A method is provided for determining in real time the course of thrombin activity in a sample of clotting blood or plasma as it appears in and disappears from the sample which comprises adding a signal substrate to said sample, said signal substrate causing a detectable signal related to the amount of conversion product formed upon reaction by the generated proteolytic activity, monitoring the signal development in time in said sample to provide a curve, and mixing said sample frequently so that clot formation occurs in a dense manner such that the majority of the sample remains fluid and that cell precipitation is inhibited, wherein said monitoring and mixing steps are repeated and performed in an alternate way.

La présente invention concerne un procédé pour déterminer en temps réel l'évolution de l'activité de la thrombine dans un échantillon de sang ou de plasma coagulé tandis qu'elle apparaît et qu'elle disparaît de l'échantillon. Ledit procédé comprend l'addition d'un substrat de signal audit échantillon, ledit substrat de signal entraînant un signal détectable lié à la quantité de produit de transformation formé pendant la réaction par l'activité protéolytique générée, le suivi de la progression du signal dans le temps dans ledit échantillon afin de fournir une courbe, et le mélange fréquent dudit échantillon de sorte que la formation de caillots d'effectue d'une manière dense de sorte que la majorité de l'échantillon reste fluide et que la précipitation des cellules soit inhibée, selon laquelle lesdites étapes de suivi et de mélange sont répétées et effectuées d'une manière alternée.

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