Method for measuring viral infectivity

Details Method for measuring viral infectivity Method for measuring viral infectivity

G - Physics

01

N

G01N 33/569 (2006.01)

Patent

CA 2259549

The instant invention addresses the need for a more accurate method of quantitating infectious viral particles in a population. The methods of the instant invention are based on the unexpected and surprising result that flow cytometry analysis of cells infected at a low ratio of particle number to cell number yields a more accurate measurement of infectious virus titer than traditional titration methods.

La présente invention répond à la nécessité d'un procédé plus précis permettant de mesurer des particules virales infectieuses dans une population. Les procédés de l'invention sont fondés sur la découverte inattendue et surprenante que l'analyse par cytométrie de flux de cellules infectées à un rapport bas entre un nombre de particules et un nombre de cellules permet un mesurage plus précis du titre du virus infectieux que les méthodes de titrage classiques.

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