Method for microbial production of amino acids of the...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/52 (2006.01) C12N 9/00 (2006.01) C12P 13/06 (2006.01) C12P 13/08 (2006.01) C12P 13/14 (2006.01) C12P 13/20 (2006.01)

Patent

CA 2305577

The invention relates to a method for microbial production of amino acids of the aspartate and/or glutamate family in which the pyruvate-carboxylase activity is increased by genetically changing the enzyme and/or the pyruvate- carboxylase gene expression of a microorganism which produces the corresponding amino acid. In addition, the invention relates to a pyruvate- carboxylase gene and additional agents which can be used in the inventive method.

L'invention concerne un procédé de préparation microbienne d'aminoacides de la famille de l'aspartate et/ou du glutamate, selon lequel l'activité pyruvate-carboxylase est améliorée par modification génétique de l'enzyme et/ou l'expression génique pyruvate-carboxylase d'un micro-organisme produisant l'aminoacide correspondant. L'invention concerne en outre un gène pyruvate-carboxylase, ainsi que d'autres agents utilisables dans le cadre de l'application dudit procédé.

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