Method for modifying cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/24 (2006.01) C12N 5/073 (2010.01) C12N 5/0735 (2010.01) C12N 5/0775 (2010.01) C12N 5/0789 (2010.01) C12N 9/10 (2006.01) C12Q 1/34 (2006.01) C12Q 1/48 (2006.01) C12P 1/00 (2006.01)

Patent

CA 2712425

The invention describes specific sialylated structures present on human stem cells and cell populations derived thereof. The invention is especially directed to methods to control the status of stem cells by changing sialylation and/or fucosylation levels of the cells. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered. The control methods are preferably mass spectrometric methods.

L'invention concerne des structures sialylées spécifiques présentes sur des cellules souches humaines ainsi que des populations cellulaires dérivées de ces dernières. L'invention concerne notamment des méthodes de contrôle de l'état de cellules souches par modification des niveaux de sialylation et/ou fucosylation des cellules. L'invention concerne également de nouvelles cellules souches, dont la glycosylation a été spécifiquement modifiée. Les méthodes de contrôle sont de préférence des méthodes de spectrométrie de masse.

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