Method for modifying lignin composition and increasing in...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/10 (2006.01)

Patent

CA 2404104

Methods for transforming forage legumes or woody plants with a DNA construct comprising at least one open reading frame encoding for a caffeoyl CoA 3-O- methyltransferase enzyme or a Medicago sativa caffeic acid 3-O- methyltransferase enzyme or a fragment thereof in either a sense or antisense orientation under a lignification-associated tissue specific promoter have been found, resulting in the down-regulation of the corresponding homologous gene either through antisense inhibition or sense suppression, as well as reduced lignin content and modified lignin composition in the transgenic plants. The expression of the caffeoyl CoA 3-O-methyltransferase transgene produces an increased syringyl lignin to guaiacyl lignin ratio in the transformed plant, and greatly improved forage digestibility.

L'invention porte sur des procédés de transformation des légumineuses fourragères ou des plantes ligneuses au moyen d'un produit de recombinaison d'ADN comprenant au moins un cadre de lecture ouvert codant pour une enzyme CoA 3-O-méthyltransférase caféoyl ou une enzyme 3-O-méthyltransférase de l'acide caféique de Medicago sativa ou un fragment de celle-ci dans une orientation sens ou antisens sous un promoteur spécifique d'un tissu associé à la lignification. Ces procédés donnent lieu à la régulation progressive du gène homologue correspondant soit par inhibition de l'antisens, soit par suppression du sens, ainsi qu'une teneur réduite en lignine et une composition de lignine modifiée dans les plantes transgéniques. L'expression du transgène de 3-O-méthyltransférase CoA caféoyl produit une plus grande quantité de lignine syringyl par rapport à la lignine guaiacyl de la plante transformée, et une bien meilleure digestibilité du fourrage.

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