Method for modifying non-starch carbohydrate material using...

C - Chemistry – Metallurgy – 12 – P

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C12P 7/10 (2006.01) C12P 19/02 (2006.01)

Patent

CA 2731890

A method for the modification of a material comprising a non-starch carbohydrate, which method comprises contacting said material comprising a non-starch carbohydrate with a polypeptide which has peroxidase activity and which is: a. a polypeptide comprising an amino acid sequence having at least 55% homology with an amino acid sequence set out in any one of: amino acids 21 to 513 of SEQ NO: 2; amino acids 20 to 510 of SEQ ID NO: 4; amino acids 1 to 493 of SEQ ID NO 6; or amino acids 1 to 491 of SEQ ID NO: 8 or b. a polypeptide encoded by a polynucleotide comprising the nucleotide sequence having at least 55% homology with a nucleotide sequence set out in any one of: nucleotides 61 to 1539 of SEQ ID NO: 1; nucleotides 58 to 1530 of SEQ ID NO: 3; nucleotides 1 to 1479 of SEQ ID NO: 5; or nucleotides 1 to 1473 of SEQ ID NO: 7.

L'invention porte sur un procédé pour la modification d'une matière comprenant un glucide qui n'est pas de l'amidon, lequel procédé comprend la mise en contact de ladite matière contenant un glucide qui n'est pas de l'amidon avec un polypeptide qui a une activité de peroxydase et qui est : a. un polypeptide comprenant une séquence d'acides aminés dont lhomologie est d'au moins 55 % avec une séquence d'acides aminés parmi l'une quelconque des séquences suivantes : les acides aminés 21 à 513 de SEQ NO: 2 ; les acides aminés 20 à 510 de SEQ ID NO: 4 ; les acides aminés 1 à 493 de SEQ ID NO: 6 ; ou des acides aminés 1 à 491 de SEQ ID NO: 8 ou b. un polypeptide codé par un polynucléotide comprenant la séquence nucléotidique dont lhomologie est d'au moins 55 % avec une séquence nucléotidique parmi l'une quelconque des séquences suivantes : les nucléotides 61 à 1539 de SEQ ID NO: 1 ; les nucléotides 58 à 1530 de SEQ ID NO: 3 ; les nucléotides 1 à 1479 de SEQ ID NO: 5 ; ou les nucléotides 1 à 1473 de SEQ ID NO: 7.

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