Method for mutagenesis by oligonucleotide-directed repair of...

Details Method for mutagenesis by oligonucleotide-directed repair of... Method for mutagenesis by oligonucleotide-directed repair of...

C - Chemistry, Metallurgy

12

N

195/1.14

C12N 15/11 (2006.01) C12N 15/10 (2006.01)

Patent

CA 1315223

Abstract A method for double-strand break repair and for site-directed mutagenesis in which a linear DNA molecule (target) and an oligonucleotide are cocultivated in a host cell. The oligonucleotide may have a mutation in its nucleotide sequence. The nucleotide sequence is, except for any mutation, homologous to that of the target and overlaps the nucleotide sequence at the ends of the target.

541442

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