Method for non-competitive binding assays

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) G01N 33/537 (2006.01) G01N 33/543 (2006.01) G01N 33/74 (2006.01) G01N 33/00 (2006.01)

Patent

CA 2117597

A novel non-competitive immunoassay technique has been developed which not only improves sensitivity, but also is convenient and less susceptible to interfering factors. It is compatible with existing instruments and is an assay that can be run in one test tube. The analyte is reacted with labeled specific binder, after which the mixture is reacted with (1) an insoluble material attached to an analyte derivative and (2) a solid phase carrying a binder. The solid phase is then separated, and the label attached to the solid phase is measured.

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