C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2340622
The present invention relates to a novel method for non-radioactive detection of membrane-bonded nucleic acids, including nucleic acids that, for instance, contain single nucleotide polymorphisms (SNP's), DNA arrays (cosmide, yeast, artificial chromosones (YAC's), bacterial artificial chromosones (BAC's), cDNA's, PCR fragments, oligonucleotides), RNA arrays and all nucleic acid fragments that are tranfered from gels (agarose or PAA) to membranes, including genomic DNA/ plasmid DNA fragments (southern) and mRNA's (northern). The invention also relates to a test kit to carry out said method.
L'invention concerne un nouveau procédé pour la détection non radioactive d'acides nucléiques liés à une membrane, à savoir, des acides nucléiques renfermant par exemple des polymorphismes nucléotidiques simples (SNP), des alignements d'ADN (cosmides, chromosomes artificiels de levure [YAC], des chromosomes artificiels de bactéries [BAC], ADNc, fragments de PCR, oligonucléotides), des alignements d'ARN et des fragments d'acides nucléiques qui sont transférés, à partir de gels (agarose ou PAA) sur des membranes, à savoir, des fragments d'ADN /plasmide-ADN (sud) et ARNm (nord). L'invention concerne en outre un matériel de test pour la mise en oeuvre de ce procédé.
Delbruck Sebastian
Hoehe Margret
Genprofile Ag
Kirby Eades Gale Baker
LandOfFree
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