Method for obtaining a cell population enriched in stem...

A - Human Necessities – 61 – K

Patent

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Details

A61K 35/14 (2006.01) A61K 35/28 (2006.01) G01N 1/18 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/569 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2171951

The invention provides methods for obtaining population of cells enriched in stem cells utilizing a combination of physical cell separation; lysosomotropic agents and additional positive and negative purification steps. The invention further provides a population enriched in stem cells obtained by the methods described herein.

L'invention se rapporte à des procédés de production de populations cellulaires enrichies en cellules souches et qui consistent à effectuer une séparation physique des cellules; à utiliser des agents lysosomotropes et à effectuer des étapes supplémentaires de purification selon des techniques de sélection positive et négative. L'invention se rapporte en outre à une population enrichie en cellules souches obtenues par les procédés décrits ci-dessus.

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